Kun Xu

Ph.D.; A.P.

College of Animal Science & Technology

Northwest A&F University

No.22 Xinong Road, YangLing, Shaan'xi

Tel: 17792639752

Email: xukunas@nwafu.edu.cn，252933605@qq.com，

Education:

2009-2015: Ph.D., Genetics, College of Animal Science and Technology, Northwest A&F University, China;

2005-2009: B.Sc., Bioengineering, College of Life Sciences, Northwest A&F University, Shaanxi, China.

Academic Experience:

2018-Present: Associate Professor, College of Animal Science and Technology, Northwest A&F University, China;

2015-2018: Lecturer/Post-Doctor, College of Animal Science and Technology, Northwest A&F University, China.

Research Interests:

Gene Editing Technology and Animal Molecular Breeding: animal genome editing technology, animal mitochondrial DNA editing technology, animal molecular design breeding.

Microbial Gene Engineering and Animal Health Raising: microbial genetic engineering, microbial synthetic biology, microbial cell microcapsule technology,  in vivo targeted delivery technology by whole yeast, intestinal microbes and mucosal immunity.

Teaching:

Molecular biology

Animal biochemistry

Animal biotechnology

Modern animal breeding

Animal genetic engineering technology

Principles and Techniques of Gene Editing

Animal reproduction theory and biotechnology

Selected Publications:

(1) Gene Editing Technology and Animal Molecular Breeding

[1] Ma Lixia, Xing Jiani, Li Qian, Zhang Zhiying*, Xu Kun*. Development of a universal antibiotic resistance screening reporter for improving efficiency of cytosine and adenine base editing. J Biol Chem. 2022 Jul; 298(7):102103.

[2] Lyu Ming, Wang Xu, Meng Xiangyu, Qian Hongrun, Li Qian, Ma Baoxia, Zhang Zhiying*, Xu Kun*. chi-miR-487b-3p inhibits goat myoblast proliferation and differentiation by targeting IRS1 through the RS1/PI3K/Akt signaling pathway. Int. J. Mol. Sci. 2021 Dec 23;23(1):115.

[3] Ren Chonghua#, Xu Kun#, David Jay Sega, Zhang Zhiying*. Strategies for the enrichment and selection of genetically modified cells. Trends Biotechnol. 2019 Jan; 37(1):56-71.

[4] Li Xinyi, Bai Yichun, Cheng Xinzhen, Peter Girgis Tawfek Kalds, Sun Bing, Wu Yun, Lyu Huijiao, Xu Kun*, Zhang Zhiying*. Efficient SSA-mediated precise genome editing using CRISPR/Cas9. FEBS J. 2018 Sep; 285(18):3362-75.

[4] Shao Simin, Ren Chonghua, Liu Zhongtian, Bai Yichun, Chen Zhilong, Wei Zehui, Wang Xin, Zhang Zhiying*, Xu Kun*. Enhancing CRISPR/Cas9-mediated homology directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Int J Biochem Cell Biol. 2017 Nov; 92:43-52.

[5] Ren Chonghua#, Xu Kun#, Liu Zhongtian, Shen Juncen, Han Furong, Chen Zhilong, Zhang Zhiying*. Dual-reporter surrogate systems for efficient enrichment of genetically modified cells. Cell Mol Life Sci. 2015 Jul; 72(14):2763-72.

[6] Xu Kun, Ren Chonghua, Liu Zhongtian, Zhang Tao, Zhang Tingting, Li Duo, Wang Ling, Yan Qiang, Guo Lijun, Shen Juncen, Zhang Zhiying*. Efficient genome engineering in eukaryotes using Cas9 from Streptococcus thermophilus . Cell Mol Life Sci. 2015 Jan; 72(2):383-99.

(2) Microbial Gene Engineering and Animal Health Raising

[1] Yue Feng, Du Lihong, Wang Ruyu, Han Baoquan, Zhang Xiaojun, Yao Zhangzhang, Zhang Wenqiang, Cai Chang, Zhang Zhiying*, Xu Kun*. In vivo Protein Interference Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control. Front Microbiol. 2022 Jun 14; 13:923656. Frontiers in Microbiology.

[2] Cui Jieyu, Li Yankun, Zhang Wenqiang, Qian Hongrun, Zhang Zhiying*, Xu Kun*. Alginic acid induces oxidative stress-mediated hormone secretion disorder, apoptosis and autophagy in mouse granulosa cells and ovaries. Toxicology. 2022 Feb 15;467:153099.

[3] Hafiz Muhammad Zakria, Han Baoquan, Yue Feng, Mu Lu, Fang Yuanyuan, Li Xiaoyu, Xu Kun*, Zhang Zhiying*. Significant body mass increase by oral administration of a cascade of shIL21-MSTN yeast-based DNA vaccine in mice. Biomed Pharmacother. 2019 Oct; 118:109147.

[4] Han Baoquan#, Xu Kun#,*, Liu Zhongtian, Ge Wei, Shao Simin, Li Pengcheng, Yan Nana, Li Xinyi, Zhang Zhiying*. Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus. Fish Shellfish Immunol. 2019 Jan; 84:948-54.

[5] Luo Zhixin, Wang Shanhe, Jiao Beilei, Yuan Dan, Dai Dongmei, Wang Lixian, Xu Kun*, Wang Xin*. Gene cloning and seamless site-directed mutagenesis using single strand annealing (SSA). Appl Microbiol Biotechnol. 2018 September 12; 102 (23):10119-26.

(Updated on 2022.10.25)