CURRICULUM VITAE
PERSONAL DATAName: Chengbao Wang
Gender: Male
Date of Birth: 1981.04.08
Place of Birth: Shandong, P. R. China
Nationality: P. R. China
Marital Status: Married
College of Veterinary Medicine Northwest A&F University
22 Xinong Road, Yangling, Shaanxi Province, 712100, PR China
Tel: + 86-029-87091117
Mobile phone: +86-0-18049569130
Email: wangchengbao@nwsuaf.edu.cn & 65285734@qq.com
Preventive Veterinary Medicine
2024-present: Professor, College of Veterinary Medicine Northwest A&F University, P. R. China.
2014-2024: Associate Professor, College of Veterinary Medicine Northwest A&F University, P. R. China.
2011-2013: Lecture, College of Veterinary Medicine Northwest A&F University, P. R. China.
2010-2011: Assistant Professor, Harbin Veterinary Research Institute, P. R. China.
2005-2010: Ph.D., Prevention Veterinary Science, the Graduate School of Chinese Academy of Agricultural Sciences (CAAS); Harbin Veterinary Research Institute, P. R. China. Tutors: Prof. Li Feng and Prof. Fei Xue.
2001-2005: Bachelor of Agriculture, Veterinary Science, Qingdao Agricultural University, P. R. China.
2005-present, Harbin Veterinary Research Institute, CAAS and College of Veterinary Medicine Northwest A&F University.
(1) Avian Disease Research
l Prevention, control and treatment of disease in pigeon and avain
(2) Ruminant Disease Research
l Prevention, control and treatment of disease in cattle and sheep
(3) Swine Disease Research
l Rescue of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and porcine reproductive and respiratory syndrome vaccine virus (PRRSV) infectious clones using a bacterial artificial chromosome
l Study on marker vaccine of HP-PRRSV by reverse genetic system
l Rescue of a novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit
l Recombinant PRRSV expressed porcine Interleukin-4 as a potential of PRRSV vaccine
l Recombinant PRRSV expressed porcine granulocyte macrophage colony-stimulating factor as a potential of PRRSV vaccine
l Identification of the non-essential region of non-structural protein 2 (NSP 2) of HP-PRRSV and PRRSV
l Role of body transcription-regulating sequences in regulation of gene expression and replication of PRRSV
l Isolation and identification of HP-PRRSV and PRRSV
l Study on construction the reverse genetic system of transmissible gastroenteritis virus
l Molecular epidemiology of porcine epidemic diarrhea virus, transmissible gastroenteritis virus and porcine rotavirus in China
l Diagnosis of classical and highly pathogenic porcine reproductive and respiratory syndrome virus
Molecular Biology
Gene clone and sequence analysis
Full-length virus cDNA clone construction
Virus isolation
Expression and purification of recombinant proteins
SDS-PAGE and Western Blot
Animal Tissue Culture
Cell culture
Plasmid transfection and virus rescue
Animal Experiment
Swine and mouse experiment including immunization, blood collection, challenge, virus isolation and titration.
[1] Sun, D.B., Lang, H.W., Shi, H.Y., Chen, J.F., Cui, X.C., Wang, C.B., Tong, Y.E., Feng, L., 2007.Screening and identification of B cell antigenic epitopes of porcine epidemic diarrhea virus spike glycoprotein by phage display. Progress in Biochemistry and Biophysics. 34(9):971-977. (in Chinese)
[2] Shi, H.Y., Feng, L., Chen, J.F., Sun, D.B., Cui, X.C., Wang, C.B., 2008. Development of an indirect ELISA for detection antibodies to porcine rotavirus using recombinant VP7 antigen. Chinese Journal of Preventive Veterinary Medicine 30(3):233-237. (in Chinese)
[3] Lv, M.J., Feng, L., Shi, H.Y., Chen, J.F., Sun, D.B., Shen, S.C., Cui, X.C., Wang, C.B., Fan, X.P., 2009. Eukaryotic expression and subcellular localization preliminary analysis of porcine epidemic diarrhea virus Nucleocapsid protein. Acta Veterinaria et Zootechnica Sinica 40(5):691-696. (in Chinese)
[4] Shen, S.C., Shi, H.Y., Chen, J.F., Sun, D.B., Lv, M.J., Wang, C.B., Fan, X.P., Chen, X.J., Feng, L., 2009. Expression of porcine teschovirus Swine/ CH/IMH/03 strain VP1 protein in Escherichia coli and analysis of its reactinogenicity. Chinese Veterinary Science 39(10):847-851. (in Chinese)
[5] Wang, C.B., Chen, J.F., Shi, H.Y., Qiu, H.J., Xue, F., Liu, C.M., Zhu, Y.M., Liu, S.W., Almazan, F., Enjuanes, L., Feng, L., 2010. Molecular characterization of a Chinese vaccine strain of transmissible gastroenteritis virus: mutations that may contribute to attenuation. Virus genes 40(3), 403-409.
[6] Wang, C.B., Feng, L., Chen, J.F., Shi, H.Y., Qiu, H.J., Xue, F., Zhu, Y.M., Liu, S.W., 2010. Analysis of the gene 3 region sequences of Chinese field strains of Transmissible gastroenteritis virus. Acta virologica 54(1), 61-73.
[7] Wang, C.B., Chen, J.F., Shi, H.Y., Qiu, H.J., Xue, F., Liu, S.W., Liu, C.M., Zhu, Y.M., Almazan, F., Enjuanes, L., Feng, L., 2010. Rapid differentiation of vaccine strain and Chinese field strains of transmissible gastroenteritis virus by restriction fragment length polymorphism of the N gene. Virus genes 41(1), 47-58.
[8] Chen, J.F., Wang, C.B., Shi, H.Y., Qiu, H.J., Liu, S.W., Chen, X.J., Zhang, Z.B., Feng, L., 2010. Molecular epidemiology of porcine epidemic diarrhea virus in China. Archives of virology 155(9), 1471-1476.
[9] Chen, J.F., Wang, C.B., Shi, H.Y., Qiu, H.J., Liu, S.W., Shi, D., Zhang, X., Feng, L., 2011. Complete genome sequence of a Chinese virulent porcine epidemic diarrhea virus strain. Journal of virology 85(21), 11538-11539.
[10] Shi, H.Y., Chen, J.F., Wang, C.B., Zhang Z.B., Shi, D., Feng, L.,2011. Isolation and identification of the porcine rotavirus isolate of Rotavirus A pig/China/NMTL/2009/G9P[23]. Chinese Journal of Preventive Veterinary Medicine 33(9):681-684. (in Chinese)
[11] Wang, C.B., Zhao, Q., Liang, C., Dang, L., Ma, Y.P., Gao, J.M., Li, Q.Y., Huang, B.C., Kong, N., Zhang, C., Zhou, E.M., 2012. Complete genome sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus variant. Journal of virology 86(16), 8906.
[12] Shi, H.Y., Chen, J.F., Li, H.X., Sun, D., Wang, C.B., Feng, L., 2012. Molecular characterization of a rare G9P[23] porcine rotavirus isolate from China. Archives of virology 157(10), 1897-1903.
[13] Wang, C.B., Huang, B.C., Kong, N., Li, Q.Y., Ma, Y.P., Li, Z.J., Gao, J.M., Zhang, C., Wang, X.P., Liang, C., Dang, L., Xiao, S.Q., Mu, Y., Zhao, Q., Sun, Y.N., Almazan, F., Enjuanes, L., Zhou, E.M., 2013. A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit. Veterinary research 44(1), 104.
[14] Wang, X.P., Wei, R.F., Li, Q.Y., Liu, H.L., Huang, B.C., Gao, J.M., Mu, Y., Wang, C.B., Hsu, W.H., Hiscox, J.A., Zhou, E.M., 2013. PK-15 cells transfected with porcine CD163 by PiggyBac transposon system are susceptible to porcine reproductive and respiratory syndrome virus. Journal of virological methods 193(2), 383-390.
[15] Zhao, Q., Sun, Y.N., Hu, S.B., Wang, X.J., Xiao, Y.H., Hsu, W.H., Xiao, S.Q., Wang, C.B., Mu, Y., Hiscox, J.A., Zhou, E.M., 2013. Characterization of antigenic domains and epitopes in the ORF3 protein of a Chinese isolate of avian hepatitis E virus. Veterinary microbiology 167(3-4), 242-249.
[16] Gao, J.M., Ji, P.C., Zhang, M.D., Wang, X., Li, N.B., Wang, C.B., Xiao, S.Q., Mu, Y., Zhao, Q., Du, T.F., Sun, Y.N., Hiscox, J.A., Zhang, G.P., Zhou, E.M., 2014. GP5 expression in Marc-145 cells inhibits porcine reproductive and respiratory syndrome virus infection by inducing beta interferon activity. Veterinary microbiology 174(3-4), 409-418.
[17] Xiao, S.Q., Zhang, A.K., Zhang, C., Ni, H.B., Gao, J.M., Wang, C.B., Zhao, Q., Wang, X.P., Wang, X.J., Ma, C., Liu, H.L., Li, N., Mu, Y., Sun, Y.N., Zhang, G.P., Hiscox, J.A., Hsu, W.H., Zhou, E.M., 2014. Heme oxygenase-1 acts as an antiviral factor for porcine reproductive and respiratory syndrome virus infection and over-expression inhibits virus replication in vitro. Antiviral research 110, 60-69.
[18] Yu, Y., Cai, X.H., Wang, G., Kong, N., Liu, Y., Xiao, Y.H., Zhang, C., Mu, Y., Xiao, S.Q., Zhao, Q., Wang, C.B., Zhang, G.P., Hiscox, J.A., Zhou, E.M., 2014. Anti-idiotypic antibodies reduce efficacy of the attenuated vaccine against highly pathogenic PRRSV challenge. BMC veterinary research 10, 39.
[19] Xiao, Y.H., Wang, T.T., Zhao, Q., Wang, C.B., Lv, J.H., Nie, L., Gao, J.M., Ma, X.C., Hsu, W.H., Zhou, E.M., 2014. Development of indirect ELISAs for differential serodiagnosis of classical and highly pathogenic porcine reproductive and respiratory syndrome virus. Transboundary and emerging diseases 61(4), 341-349.
[20] Li, Z.J., Wang, G., Wang, Y., Zhang, C., Wang, X.L., Huang, B.C., Li, Q.Y., Li, L.L., Xue, B.Y., Ding, P.Y., FarazSyed, S., Wang C.B.*(Corresponding author), Cai, X.H.,* Zhou, E.M.,* 2015. Rescue and Evaluation of a Recombinant PRRSV Expressing Porcine Interleukin-4. Virology Journal. 12, 185.
[21] Li Z.J., Wang G., Wang Y., Zhang C., Huang B.C., Li Q.Y., Li L.L., Xue B.Y., Ding P.Y., Cai X.H., Wang C.B.*(Corresponding author), Zhou, E.M.* 2015. Immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine GM-CSF.Vet Immunol Immunopathol.168(1-2),40-48.
[22] Xiao, S.Q., Wang, X., Ni, H.B., Li, N., Zhang, A.K., Liu, H.L., Pu, F.X., Xu, L., Gao, J.M., Zhao, Q., Mu, Y., Wang, C.B., Sun, Y.N., Du, T.F., Xu, X., Zhang, G.P., Hiscox, J.A., Goodfellow, I.G., Zhou, E.M., 2015. MicroRNA miR-24-3p Promotes Porcine Reproductive and Respiratory Syndrome Virus Replication through Suppression of Heme Oxygenase-1 Expression. Journal of virology 89(8), 4494-4503.
[23] Yu, Y., Wang, G., Li, Q.Y., Du, Y.K., Du, T.F., Mu, Y., Xiao, S.Q., Zhao, Q., Wang, C.B., Sun, Y.N., Xu, X., Zhang, G.P., Hsu, W.H., Cai, X.H., Zhou, E.M., 2015. Single-chain anti-idiotypic antibody retains its specificity to porcine reproductive and respiratory syndrome virus GP5. Immunology letters 163(1), 8-13.
[24] Li, Q.Y., Wang X.P., Wang C.B., Yu Y, Wang G, Gao J.M., Liu H.L., Xie H, Huang B.C., Li Z.J., Kong N, Zhang G.P., Hsu W.H., Zhou E.M., 2016. Intracellular expression of an anti-idiotypic antibody single-chain variable fragment reduces porcine reproductive and respiratory syndrome virus infection in MARC-145 cells. Antiviral therapy 21(2), 161-170.
[25] Lv, H.F., Dong, W., Qian, G., Wang, J., Li, X., Cao, Z., Lv, Q.Z., Wang, C.B., Guo, K.K., Zhang, Y.M., 2017. Us10, a novel Npro-interacting protein, inhibits classical swine fever virus replication. Journal of General Virology, 98(7), 1679-1692.
[26] Lv, H.F., Dong, W., Cao, Z., Li, X.M., Wang, J., Qian, G., Lv, Q.Z., Wang, C.B., Guo, K.K., Zhang, Y.M., 2017. Traf6 is a novel ns3-interacting protein that inhibits classical swine fever virus replication. Scientific Reports, 7(1), 6737.
[27] Dong, W., Lv, H.F., Wang, Y.F., Li, X.M., Li, C., Wang, L., Wang, C.B., Guo, K.K., Zhang, Y.M., 2017. The effect of classical swine fever virus ns5a and ns5a mutants on oxidative stress and inflammatory response in swine testicular cells. Research in Veterinary Science, 112, 89-96.
[28] Lin, J.H., Wang, C.B., Zhang, L.X., Wang, T., Liang, W.L., Li, C., Qian, G., Ouyang, Y.L., Guo, K.K., Zhang, Y.M., 2017. Rab5 enhances classical swine fever virus proliferation and interacts with viral ns4b protein to facilitate formation of ns4b related complex. Frontiers in Microbiology, 8, 1468.
[29] Lin, J.H., Wang, C.B., Liang, W.L., Zhang, J., Zhang, L.X., Lv, H.F., Dong, W., Zhang, Y.M., 2017. Rab1a is required for assembly of classical swine fever virus particle. Virology, 514, 18-29.
[30] Wang, C.B., Han, M., Gao, Y.J., Gao, H., Guo, K.K., Almazan, F., Sola, I., Enjuanes, L., Zhang, Y.M.*, Abrahamyan, L.*, 2017. Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus. Veterinary Research, 48(1), 41.
[31] Tang, Y.F., Zeng, W.W.*, Wang, Q., Wang, Y.Y., Shi, C.B., Feng, R., Gao, H., Wang, C.B.*, 2017. The Research and Application of Reverse Genetics in Reoviridae Family. Chinese Journal of Virology 33(9):950-958. (in Chinese).
[32] Gao, H.#, Zhang, M.H.#, Feng, R., Tang, Y.F., Zhang, H., Chen, C., Lv, T., Wang, C.B.*, Yang, Y.T.*, 2018. Molecular cloning and prokaryotic expression of Penton gene of Fowl Adenovirus Group I serotype four. Journal of Progress in Veterinary Medicine 39(05):1-5. (in Chinese).
[33] Feng, R., Gao, Y.J., Zhang, M.H., Gao, H., Zhang, H., Lv, T., Chen, C., Wang, C.B.*, Yang, Y.T.*, 2018. Cloning and sequence analysis of 33K、Fiber-1、Fiber-2 and pⅢa genes of Fowl Adenovirus type 4 isolated from Shaanxi province. Journal of Progress in Veterinary Medicine 39(04):7-13. (in Chinese).
[34] Tang, Y.F., Zeng, W.W.*, Wang, Q., Wang, Y.Y., Shi, C.B., Feng, R., Gao, H., Wang C.B.*, 2018. Preparation and Specificity of Polyclonal Antibody against Grass carp reovirus Type II VP6 Protein. Freshwater Fisheries 48(01):9-14. (in Chinese).
[35] Wang H.R., Gao H., Jiang Z.W., Shi L.B., Zhao P.W., Zhang Y.M., Wang C.B.*, 2022. Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in Northern China. BMC Genomics 23(1):290.
[36] Zhuang H., Sun L.L., Wang X.B., Xiao M., Zeng L., Wang H.R., Yang H.F., Lin F., Wang C., Qin L.T., Wang C.B.*, 2022. Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus strains circulating in China from 2020 to 2021. BMC Veterinary Research 18(1):392.
[37] Mao Q., Ma S.M., Schrickel P.L., Zhao P.W., Wang J.Y., Zhang Y.H., Li S.Y., Wang C.B.*, 2022. Review detection of Newcastle disease virus. Frontiers in Veterinary Science, 9, 936251.
[38] Zhang Y.H., Lu Z., Zhao H.Y., Li S. Y., Zhuang H., Wang J., Li R.C., Zheng W.B., Zhu H.W., Xie P., Hu Y.B., Zhou C.Y., Mao Q., Sun L.L., Li S.S., Wang W.H., Wang F., Pan W., Wang C.B.*, 2024. Antimicrobial resistance and genomic characterization of Salmonella isolated from pigeons in China, Transbound Emerg Dis, ID 3315678
[39] Mao Q, Ma S.M., Li S.Y., Zhang Y.H., Li S.S., Wang W.H., Wang F., Guo Z.K., Wang C.B.*, 2024. PRRSV hijacks DDX3X protein and induces ferroptosis to facilitate viral replication, Vet Res 55(1):103.
We are looking to recruit MSc and PhD students from an animal husbandry or veterinary background, and would like to enrol international students with an interest in avian, ruminant, cattle and sheep diseases, as well as in preventive veterinary medicine.
