Title: Multiple gene modified pig for organ transplantation

Speaker: Dengke Pan, PhD

Time: AM 9:30-11:30, July 7, 2015 

Venue: Meeting room 107, College of Animal Science and Technology

Introduction:

Dengke Pan is an associate professor in the Institute of Animal Sciences, Chinese Academy of Agricultural Sciences. He is famous for his pioneering work of producing the first somatic cloned pig in China. Development of humanized pigs for organ transplantation is his main goal. Dr. Pan optimized somatic cell nuclear transfer, transgenic technology, genome editing and embryo chimera in pig. His group obtained many achievements. α1,3-galactosyl transferase gene konckout (GTKO) pigs were produced, which could overcome hyperacute rejection when xenotransplantation. GTKO pig-to-monkey liver transplantation was conducted, and Monkey survived for 14 days, the international longest surviving time record. Multiple gene modified pigs had been generated. Genetically-modified pigs is potential sources of islet, heart , liver and kidney for human organ transplantation.

Representative papers:

1.      Ji H, Li X, Yue S, Li J, Chen H, Zhang Z, Ma B, Wang J, Pu M, Zhou L, Feng C, Wang D, Duan J, Pan D*, Tao K, Dou K. Pig BMSCs transfected with human TFPI combat species incompatibility and regulate the human TF pathway in Vitro and in a rodent model. Cellular Physiology and Biochemistry, 2015, 36(1): 233-249

2.      Zhou Y, Lin Y, Wu X, Feng C, Long C, Xiong F, Wang N, Pan D*, Chen H. The high-level accumulation of n-3 polyunsaturated fatty acids in transgenic pigs harboring the n-3 fatty acid desaturase gene from Caenorhabditis briggsae. Transgenic Research, 2014, 23(1): 89-97

3.      Nallasamy P, Si H, Babu P, Pan D, Fu Y, Brooke E, Shah H, Zhen W, Zhu H, Liu D, Li Y, Jia Z*. Sulforaphane reduces vascular inflammation in mice and prevents TNF-α-induced monocyte adhesion to primary endothelial cells through interfering with the NF-κB pathway. Journal of Nutritional Biochemistry, 2014, 25(8): 824-833

4.      Fan N, Chen J, Shang Z, Dou H, Ji G, Zou Q, Wu L, He L, Wang F, Liu K, Liu N, Han J, Zhou Q, Pan D, Yang D, Zhao B, Ouyang Z, Liu Z, Zhao Y, Lin L, Zhong C, Wang Q, Wang S, Xu Y, Luan J, Liang Y, Yang Z, Li J, Lu C, Vajta G, Li Z, Ouyang H, Wang H, Wang Y, Yang Y, Liu Z, Wei H, Luan Z, Esteban MA, Deng H, Yang H, Pei D, Li N, Pei G, Liu L, Du Y, Xiao L, Lai L. Piglets cloned from induced pluripotent stem cells. Cell Research, 2013, 23(1): 162-166

5.      Pan D*, Zhang L, Zhou Y, Feng C, Long C, Liu X, Wan R, Zhang J, Lin A, Dong E, Wang S, Xu H, Chen H. Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer. Science in China Series C: Life Sciences, 2010, 53(4): 517-523